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recombinant human bmp4  (R&D Systems)


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    R&D Systems recombinant human bmp4
    Recombinant Human Bmp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+bmp4/Recombinant+Human+BMP-4+Protein/pm42014719-310-33-36
    Average 97 stars, based on 1145 article reviews
    recombinant human bmp4 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 10 4 cells cm −2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO 2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL −1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an additional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL −1 human recombinant VEGF-A 165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10 –4 M monothioglycerol (Sigma-Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA), 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems), and then cultured in ultra-low-attachment 6-well plate (Corning) in 5% CO 2 /5% O 2 . .. After 24 hours, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/mL bFGF (R&D Systems) were added into the wells.

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM Glutamax (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10 -4 M monothioglycerol (Sigma-Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA)), 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning) in 5%CO 2 /5% O 2 . .. After 24 h, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/mL bFGF (R&D Systems) were added into the wells.

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 104 cells cm−2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL−1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an addi- tional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL−1 human recombinant VEGF-A165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.

    Article Title: Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart
    Article Snippet: Briefly, undifferentiated hiPSCs were detached from feeder cells and dissociated into single cells by 8-min incubation with Accumax (Innovative Cell Technologies, Inc, San Diego, CA). .. The cells were suspended in StemPro34 medium complemented with 2 mM L-glutamine, 50 μg/mL ascorbic acid, 4 × 104 M monothioglycerol, 150 μg/mL transferrin, 10 μM Y-27632 (WAKO), 2 ng/mL human recombinant BMP4 (R&D Systems Minneapolis, MN), and 0.5% Matrigel (Corning) and were cultured for 24 hours. .. On day 1, the media were changed to those supplemented with human recombinant activin A (R&D Systems), BMP4, and bFGF (R&D Systems).

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10–4 M monothioglycerol (Sigma- Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA), 10 μM Y- 27632 and 2 ng/mL human recombinant BMP4 (R&D Systems), and then cultured in ultra- low- attachment 6- well plate (Corning) in 5% CO2/5% O2. .. After 24 hours, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/ mL bFGF (R&D Systems) were added into the wells.

    Article Title: Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart
    Article Snippet: Briefly, undifferentiated hiPSCs were detached from feeder cells and dissociated into single cells by 8-min incubation with Accumax (Innovative Cell Technologies, Inc, San Diego, CA). .. The cells were suspended in StemPro34 medium complemented with 2 mM L-glutamine, 50 μg/mL ascorbic acid, 4 × 104 M monothioglycerol, 150 μg/mL transferrin, 10 μM Y-27632 (WAKO), 2 ng/mL human recombinant BMP4 (R&D Systems Minneapolis, MN), and 0.5% Matrigel (Corning) and were cultured for 24 hours. .. On day 1, the media were changed to those supplemented with human recombinant activin A (R&D Systems), BMP4, and bFGF (R&D Systems).

    Recombinant:

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 10 4 cells cm −2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO 2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL −1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an additional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL −1 human recombinant VEGF-A 165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10 –4 M monothioglycerol (Sigma-Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA), 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems), and then cultured in ultra-low-attachment 6-well plate (Corning) in 5% CO 2 /5% O 2 . .. After 24 hours, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/mL bFGF (R&D Systems) were added into the wells.

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM Glutamax (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10 -4 M monothioglycerol (Sigma-Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA)), 10 μM Y-27632 and 2 ng/mL human recombinant BMP4 (R&D Systems) and then cultured in ultra-low attachment 6 well plate (Corning) in 5%CO 2 /5% O 2 . .. After 24 h, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/mL bFGF (R&D Systems) were added into the wells.

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 104 cells cm−2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL−1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an addi- tional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL−1 human recombinant VEGF-A165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.

    Article Title: BMP9 Modulates IL-33 Signaling to Mitigate EndMT in Pulmonary Arterial Hypertension
    Article Snippet: .. Human recombinant BMP4 (314-BP-010/CF), BMP6 (507-BP-020/CF), BMP9 (3209-BP-010/CF), BMP10 (2926-BP-025/CF), activin A (338-AC-010/CF), TGF-b1 (240-B-010/CF), and recombinant human soluble ST2/IL-33R Fc (523-ST-100) were obtained from R&D Systems. .. Human recombinant IL-33 (CYT-425) was purchased from Prospec, and LDN-193189 (6053), a selective BMP signaling inhibitor targeting ALK1/2/3 and 6 (Activin Receptor-Like Kinase 1/2/3 and 6) other kinases, was purchased from Tocris ( Table S1 ).

    Article Title: Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart
    Article Snippet: Briefly, undifferentiated hiPSCs were detached from feeder cells and dissociated into single cells by 8-min incubation with Accumax (Innovative Cell Technologies, Inc, San Diego, CA). .. The cells were suspended in StemPro34 medium complemented with 2 mM L-glutamine, 50 μg/mL ascorbic acid, 4 × 104 M monothioglycerol, 150 μg/mL transferrin, 10 μM Y-27632 (WAKO), 2 ng/mL human recombinant BMP4 (R&D Systems Minneapolis, MN), and 0.5% Matrigel (Corning) and were cultured for 24 hours. .. On day 1, the media were changed to those supplemented with human recombinant activin A (R&D Systems), BMP4, and bFGF (R&D Systems).

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The cells were suspended in 1.5 mL cardiomyocyte differentiation media (CDM), consisting of StemPro34 medium (Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific), 50 μg/mL ascorbic acid, 4 × 10–4 M monothioglycerol (Sigma- Aldrich), 150 μg/mL transferrin (Roche), and with Matrigel (Cat#354277, Corning, NY, USA), 10 μM Y- 27632 and 2 ng/mL human recombinant BMP4 (R&D Systems), and then cultured in ultra- low- attachment 6- well plate (Corning) in 5% CO2/5% O2. .. After 24 hours, 1.5 mL CDM with 6 ng/mL human recombinant activin A (R&D Systems) and 5 ng/ mL bFGF (R&D Systems) were added into the wells.

    Article Title: Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart
    Article Snippet: Briefly, undifferentiated hiPSCs were detached from feeder cells and dissociated into single cells by 8-min incubation with Accumax (Innovative Cell Technologies, Inc, San Diego, CA). .. The cells were suspended in StemPro34 medium complemented with 2 mM L-glutamine, 50 μg/mL ascorbic acid, 4 × 104 M monothioglycerol, 150 μg/mL transferrin, 10 μM Y-27632 (WAKO), 2 ng/mL human recombinant BMP4 (R&D Systems Minneapolis, MN), and 0.5% Matrigel (Corning) and were cultured for 24 hours. .. On day 1, the media were changed to those supplemented with human recombinant activin A (R&D Systems), BMP4, and bFGF (R&D Systems).

    Concentration Assay:

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 10 4 cells cm −2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO 2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL −1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an additional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL −1 human recombinant VEGF-A 165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.

    Article Title: High-Purity Production of Endothelial Cells from Human Pluripotent Stem Cells
    Article Snippet: Cells were seeded at a density of 5.3 × 104 cells cm−2 onto Matrigel-coated wells of Nunc Cell-Culture Treated Multidishes (Thermo Fisher Scientific). .. The cells were cultured in a humidified incubator at 37°C with 5% CO2 for 24 h. Subsequently, the medium was replaced daily for 3 days with differentiation medium 1, consisting of DMEM/F12 (Merck KGaA) supplemented with 25 ng/mL human recombinant BMP4 (R&D Systems, Minneapolis, MN, USA), 8–16 μM CHIR99021 (concentration adjusted based on cell confluency and morphology; ReproCELL, Kanagawa, Japan), 50 ng mL−1 human recombinant bFGF (Fujifilm Wako), 1% (v/v) B27 supplement (Thermo Fisher Scientific), 1% (v/v) GlutaMax Supplement (Thermo Fisher Scientific), and 1% (v/v) penicillin/streptomycin. .. Following this, cells were cultured for an addi- tional 3 days with daily medium changes using differentiation medium 2, composed of StemPro-34 SFM (Thermo Fisher Scientific) supplemented with 100 ng mL−1 human recombinant VEGF-A165 (Fujifilm Wako), 10 μM DAPT (AdipoGen Life Sciences, Inc., San Diego, CA, USA), 2 μM Forskolin (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 1% (v/v) GlutaMax Supplement, and 1% (v/v) penicillin/streptomycin.



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